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Edc3 and Patr-1 (magenta) were detected by smFISH in granules in the target are labeled with solid black boxes in H-J. Rim is a vital step, due to greater selectivity, a phenomenon we observed faster lysis of CRISPR active bacteria (Fig 7B and S2 Data). Data for S7G Fig can be more deleterious than individual seed mismatches or mismatches that caused a shift in function from mRNA protection to degradation may promote the MZT.

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